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Measures Cholesterol Concentration. Coral Clinical Systems has answered these concerns of the busy laboratory professional by engineering the “Flexipour”reagent system which comprises of individual liquid stable components, which when mixed together in recommended ratios generate a dependable liquid stable working reagent. Depending on the usage, individual reagents may be poured at one go or flexibly in smaller volumes. Cholesterol is the main lipid found in blood, bile and brain tissues. It is the main lipid associated with arteriosclerotic vascular diseases. It is required for the formation of steroids and cellular membranes. The liver metabolizes the cholesterol and it is transported in the blood stream by lipoproteins. Increased levels are found in hypercholesterolaemia, hyperlipidaemia, hypothyroidism, uncontrolled diabetes nephrotic syndrome, and cirrhosis. Decreased levels are found in malabsorption malnutrition, hyperthyroidism, anemias and liver diseases Cholesterol esterase hydrolyses esterified cholesterols to free cholesterol. The free cholesterol is oxidised to form hydrogen peroxide which further reacts with phenol and amino antipyrine by the catalytic action of peroxidase to form a red coloured quinonimine dye complex. Intensity of the colour formed is directly proportional to the amount of cholesterol present in the sample