Glycosylated Haemoglobin

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Measures Level of Glycoselated Haemoglobin.This is a colirometric laboratory reagent which measures the enzyme alkaline phosphatase in the human body by the colorometricmethod.Coral Clinical Systems has answered these concerns of the busy laboratory professional by engineering the “Flexipour”reagent system which comprises of individual liquid stable components, which when mixed together in recommended ratios generate a dependable liquid stable working reagent. Depending on the usage, individual reagents may be poured at one go or flexibly in smaller volumes.Glycosylated hemoglobin ( GHb ) has been defined operationally as the fast fraction hemoglobins HbA1 (Hb A1a,A1b,A1c ) which elute first during column chromatography. The non – glycosylated hemoglobin.which consists of the bulk of hemoglobin,has been designated HbAo.A hemolysed preparation of whole blood is mixed continuously for 5 minutes with a weakly binding cation-exchange resin. The labile fraction is eliminated during the hemolysate prepration and during the binding. During this mixing, HbAo binds to the ion exchange resin leaving GHb free in the supernatant. After the mixing period, a filter separator is used to remove the resin from the supernatant . The percent glycosylated hemoglobin is determined by measuring absorbances of the glycosylated hemoglobin ( GHb) fraction