Lipase

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Measures Lipase. Coral Clinical Systems has answered these concerns of the busy laboratory professional by engineering the “Flexipour” reagent system which comprises of individual liquid stable components, which when mixed together in recommended ratios generate a dependable liquid stable working reagent. Depending on the usage, individual reagents may be poured at one go or flexibly in smaller volumes. Lipases are enzymes which hydrolyse glycerol esters of long chain fatty acids. The enzyme and its cofactor, colipase is produced in the pancreas, lipase being also secreted in small amounts by the salivary glands as well as by the gastric, pulmonary and intestinal mucosa. Bile acids and colipase form micellar complexes with the lipids and bind lipase on the substrate water interface. Determination of Lipase is used for investigation of pancreatic disorders. In acute pancreatitis the lipase concentrations rise 2-50 fold the upper reference limit within 4-8 hours after beginning of abdominal pain peaking at 24 hours and decreasing within 8-14 days. Elevated Lipase values can also be observed in chronic pancreatitis and obstruction of the pancreatic duct. Pancreatic Lipase catalyses the hydrolysis of Tirolean, in the presence of colipase, to form monoglycerides and fatty acids. The rate of decrease in turbidity measured at 340nm, is proportional to the lipase activity. The activities of other lipases in the serum are inhibited by the cholic acid salts in the reagent