SGOT

For more information see these pdf files—-  SGOT-1    SGOT-2    (rt click open in new tab)

Measures SGOT concentration.Coral Clinical Systems has answered these concerns of the busy laboratory professional by engineering the “Flexipour”reagent system which comprises of individual liquid stable components, which when mixed together in recommended ratios generate a dependable liquid stable working reagent. Depending on the usage, individual reagents may be poured at one go or flexibly in smaller volumes. SGOT is an enzyme found mainly in heart muscle, liver cells, skeletal muscle and kidneys. Injury to these tissues results in the release of the enzyme in blood stream. Elevated levels are found in myocardial infarction, Cardiac operations, Hepatitis, Cirrhosis, acute pancreatitis, acute renal diseases, primary muscle diseases. Decreased levels may be found in pregnancy, Beri Beri and diabetic ketoacidosis.SGOT converts L-Aspartate and a Ketoglutarate to Oxaloacetate and Glutamate. The Oxaloacetate formed reacts with 2,4,Dinitrophenyl hydrazine to produce a hydrazone derivative, which in an alkaline medium produces a brown coloured complex whose intensity is measured. The reaction does not obey Beer’s law and hence a calibration curve is plotted using a Pyruvate standard. The activity of SGOT (ASAT) is read off this calibration curve.