Measures SGPT concentration.Coral Clinical Systems has answered these concerns of the busy laboratory professional by engineering the “Flexipour”reagent system which comprises of individual liquid stable components, which when mixed together in recommended ratios generate a dependable liquid stable working reagent. Depending on the usage, individual reagents may be poured at one go or flexibly in smaller volumes. SGPT is found in a variety of tissues but is mainly found in the liver. Increased levels are found in hepatitis, cirrhosis, obstructive jaundice and other hepatic diseases. Slight elevation of the enzymes is also seen in myocardial infarction.SGPT converts L-Alanine and a Ketoglutarate to Pyruvate and Glutamate. The Pyruvate formed reacts with 2,4,Dinitrophenyl hydrazine to produce a hydrazone derivative, which in an alkaline medium produces a brown coloured complex whose intensity is measured. The reaction does not obey Beer’s law and hence a calibration curve is plotted using a Pyruvate standard. The activity of SGPT (ALAT) is read off this calibration curve.