Thrombin clot enhancer kit. Serum is commonly used as specimen for analysis in clinical laboratories, Serum is obtained by collecting whole blood in plain tubes or tubes coated with clot activators. The serum is then separated, after complete clotting takes place. The clotting of blood may take about 20 – 30 minutes, depending on the method used, serum is then separated from the clot for testing. On several occasions there is a delay in separating the serum from the clot. ln hospitals, outpatient clinics and collection centers, transportation of specimens from a phlebotomy site to laboratory sometimes takes longer. During a prolonged contact time between serum and clot, both biological activity of cells and trans-membrane diffusion can change the concentrations of certain analytes in the serum. Prolonged contact of serum with the clot can cause pre-analytical variations. The optimum time interval between sample collection and separation of serum from the clot should be long enough to allow complete clot formation but be shorter than the time in which a significant change in test result is induced by serum-clot contact, NCCLS procedures for handling and processing of blood specimens recommends that the serum or plasma should be physically separated from contact with cells as soon as possible, unless conclusive evidence indicates that longer contact times do not contribute to result inaccuracy. Fastrak clot activator reagent is intended to be used for activating the rapid clotting of whole blood to separate serum faster.Fastrak, Thrombin Reagent, activates the clotting mechanism and rapidly clots whole blood, within one minute. The serum can then be separated immediately for use as specimen.The working Fastrak Reagent is not very stable at elevated temperatures and should be stored back at 2-80 C immediately, after using the required amount of reagent, to avoid deterioration.