Measures Iron + TIBC.Coral Clinical Systems has answered these concerns of the busy laboratory professional by engineering the “Flexipour”reagent system which comprises of individual liquid stable components, which when mixed together in recommended ratios generate a dependable liquid stable working reagent. Depending on the usage, individual reagents may be poured at one go or flexibly in smaller volumes. Iron found in blood is mainly present in the hemoglobin of the RBC’s. It’s role in the body is mainly in the transportation of oxygen and cellular oxidation. Iron is absorbed in the small intestine, and bound to a globulin in the plasma, called transferrin,and transported to the bone marrow for the formation of hemoglobin. Increased serum levels are found in hemolytic anemias, hepatitis, lead and iron poisoning. Decreased serum levels are found in anemias caused by iron deficency due to insufficient intake or absorption of iron, chronic blood loss, late pregnancy and cancer. Increase in TIBC is found in Iron defecient anemias and pregnancy. Decrease in TIBC is found in hypoproteinemia, hemolytic /pernicious / sicklecell anemias, inflammatory diseases and cirrhosis.Iron, bound to Transferrin, is released in an acidic medium and the Ferric ions are reduced to Ferrous ions. The Fe (II) ions react with Ferrozine to form a violet coloured complex. Intensity of the complex formed is directly proportional to the amount of Iron present in the sample. For TIBC, the serum is treated with excess of Fe (II) to saturate the iron binding sites on transferrin. The excessFe (11) is adsorbed and precipitated and the Iron content in the supernatant is measured to give the TIBC.